Fig. 4From: Interference of pseudorabies virus infection on functions of porcine granulosa cells via apoptosis modulated by MAPK signaling pathwaysPRV infection impaired steroidogenesis in porcine ovarian granulosa cells. Porcine granulosa cells were infected with PRV at titers 5, 10 and 50 TCID50 for 1.5 h and then cultured for 36 h, steroid hormones in the cell culture medium were measured by radioimmunoassay, and the expression of steroidogenesis-related enzymes in cells were detected by qPCR. (A) Progesterone (P4) level. (B) Estradiol (E2) level. (C) Expression of CYP19A1, 17β-HSD, CYP11A1, 3β-HSD and StAR mRNA. Expression of the target genes were normalized to the corresponding GAPDH level. Data were mean ± SEM (n = 3). Significant differences between Mock- and PRV-infection are indicated by * P < 0.05, ** P < 0.01Back to article page