Fig. 2From: Interference of pseudorabies virus infection on functions of porcine granulosa cells via apoptosis modulated by MAPK signaling pathwaysPRV infection inhibited cell viability and Ki67 level in porcine ovarian granulosa cells. Porcine granulosa cells were infected with PRV suspension (5, 10 and 50 TCID50) for the indicated time points. (A) Cell viability was detected by MTS assay. Data represented as mean ± SEM (n = 3); Asterisks indicate a significant difference compared to the Mock group. **P < 0.01. (B) PRV infection (50 TCID50) caused obvious cytopathic effects in granulosa cells at 24 h post infection. (C) Expression of Ki67 in granulosa cells following PRV infection at 36 h by IFA. (D) Quantification of the fluorescence signal by Image J. Data show as mean ± SEM (n = 3); * P < 0.05, ** P < 0.01Back to article page