Fig. 6From: The artificial amino acid change in the sialic acid-binding domain of the hemagglutinin neuraminidase of newcastle disease virus increases its specificity to HCT 116 colorectal cancer cells and tumor suppression effectThe susceptibility of colorectal cancer cells to rNDV or S519G mutant rNDV infections. MTT assays of rNDV and S519G mutant rNDV on colorectal cancer cells (HCT 116 and HT-29) colorectal normal cells (CCD-18Co), and various cancer cells (T98G; glioblastoma, A549; lung cancer). Before one day, the cells were cultured in 96-well tissue culture plates and infected with several titers (1 MOI) of rNDV or S519G mutant rNDV viruses. For negative control, the cells were grown in a culture medium. At 96 h post-infection, MTT assays were performed Cell Proliferation Assay kit. (n = 4, error bar: SEM, *p < 0.05, **p < 0.01, ***p < 0.001 determined by the Student’s t-test). MOI; multiplicity of infection, rNDV; recombinant NDVBack to article page