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Fig. 5 | Virology Journal

Fig. 5

From: Down-regulated TAB1 suppresses the replication of Coxsackievirus B5 via activating the NF-κB pathways through interaction with viral 3D polymerase

Fig. 5

The 90-96aa domain of TAB1 is responsible for the function. A The diagram of TAB1 protein structure; B Interaction between TAB1 and 3D polymerase as predicted by Pymol; C pcDNA3.1-3D-2Flag or pcDNA3.1- TAB1-ALA-6His (pcDNA3.1 as the control) was transfected into RD cells. Cells were harvested at 24 h post-transfection and lysed. Lysates were subjected to anti-Flag immunoprecipitation and analyzed by immunoblotting; D–E pcDNA3.1- TAB1(69–334) or pcDNA3.1- TAB1- ALA (pcDNA3.1 as the control) was transfected into RD cells, then infected CVB5. Cells were harvested at 24 h.p.i.. The expression of CVB5 VP1 in cell lysates was analyzed by RT-qPCR (D) and Western blotting (E); F pcDNA3.1- TAB1, pcDNA3.1- TAB1(69–334), or pcDNA3.1- TAB1-ALA-6His (pcDNA3.1 as the control) was transfected into RD cells, and then infected CVB5. Cells were harvested at 24 h.p.i.. The expressions of NF-κB pathway proteins in cell lysates were analyzed by Western blotting. Data are represented as mean ± SD. **P ≤ 0.01

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Virology Journal

ISSN: 1743-422X