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Fig. 3 | Virology Journal

Fig. 3

From: Down-regulated TAB1 suppresses the replication of Coxsackievirus B5 via activating the NF-κB pathways through interaction with viral 3D polymerase

Fig. 3

TAB1 inhibits CVB5 replication. A–C si-TAB1 (siNC as the control) was transfected into RD cells, then infected CVB5. Cells were harvested at 24 h.p.i.. The expression of CVB5 VP1 in cell lysates was analyzed by RT-qPCR (A) and Western blotting (B); Supernatant was collected for TCID50 measurement (C); (D–F) pcDNA3.1-TAB1 plasmid (pcDNA3.1 as the control) was transfected into RD cells, then infected CVB5. Cells were harvested at 24 h.p.i.. The expression of CVB5 VP1 in cell lysates was analyzed by RT-qPCR (D) and Western blotting (E); Supernatant was collected for TCID50 measurement (F); G–I pcDNA3.1-TAB1 (pcDNA3.1 as the control) was transfected into RD cells, then infected EV71 (MOI = 0.1). Cells were harvested at 24 h.p.i.. The expression of EV71 VP1 in cell lysates was analyzed by RT-qPCR (G) and Western blotting (H); Supernatant was collected for TCID50 measurement (I). Data are represented as mean ± SD. **P ≤ 0.01

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Virology Journal

ISSN: 1743-422X