Skip to main content
Fig. 2 | Virology Journal

Fig. 2

From: Down-regulated TAB1 suppresses the replication of Coxsackievirus B5 via activating the NF-κB pathways through interaction with viral 3D polymerase

Fig. 2

CVB5 3D polymerase down-regulates TAB1 expression through the lysosomal pathway. pcDNA3.1-3D-2Flag (pcDNA3.1 as the control) was transfected into RD cells and harvested at 24 h post-transfection. A Lysates were subjected to anti-TAB1 immunoprecipitation and analyzed by immunoblotting; B Lysates were subjected to anti-Flag immunoprecipitation and analyzed by immunoblotting; C The co-localization of 3D polymerase and TAB1 analyzed by confocal assay; D Cells maintained for 24 h in the presence of the autophagy inhibitor (3MA), lysosomal inhibitor (CQ or Baf-A1), and protease inhibitor (MG132). The expression of TAB1 in cell lysates was analyzed by Western blotting. The numbers are the level of expression compare to the controls

Back to article page

Virology Journal

ISSN: 1743-422X