Table 6 Key resources and reagents
From: In-house ELISA protocols for capsid p24 detection of diverse HIV isolates
Type | Reagent | Source | Identifier |
|---|---|---|---|
Antibodies | Mouse Anti-HIV Gag CA-p24 Capture Antibody | R&D Systems | 844,721, DY7360-05 |
Biotinylated Mouse Anti-HIV Gag CA-p24 Detection Antibody | R&D Systems | 844722, DY7360-05 | |
Diluent solution, Blocking buffer | PBS tablets | Gibco | 18912-014 |
Tween 20 | Merck | 822184 | |
BSA | Roche | 10735086001 | |
Triton X-100 | Fisher | BP151-500 | |
Protein standard | HIV Gag CA-p24 | Aalto Bio Reagents | AG 6054 |
Conjugate | Streptavidin-HRP | R&D Systems | 893975, DY7360-05 |
Substrate solution | Lumi-Phos HRP | Lumigen | PSA-100 |
96-well plates | Half-area white 96-well plates | Greiner | 675074 |
Washer | BioTek ELx405 Select | Agilent | |
Shaker | Microplate shaker | VWR | 444-0270 |
Plate reader | GloMax® Navigator Microplate Luminometer | Promega | GM2010 |