Table 5 Key resources and reagents for the ANG system
From: In-house ELISA protocols for capsid p24 detection of diverse HIV isolates
Type | Reagent | Source | Identifier |
|---|---|---|---|
Antibodies | HIV-CA-p24, Alkaline Phosphatase Conjugate monoclonal antibody (mAb) (detection) | Aalto Bio Reagents | BC1071-AP |
mAb anti-HIV-1 P24 (capture) | Anogen | MO-I40002D2 | |
Dilution buffer | PBS tablets | Gibco | 18912-014 |
Tween 20 | Merck | 822184 | |
Protein standard | HIV Gag CA-p24 | Aalto Bio Reagents | AG 6054 |
Substrate solution | LumiPhos Plus | Lumigen | P-7000 |
96-well plates | Half-area white 96-well plates | Greiner | 675074 |
Washer | BioTek ELx405 Select | Agilent | |
Shaker | Microplate shaker | VWR | 444-0270 |
Plate reader | GloMax® Navigator Microplate Luminometer | Promega | GM2010 |