Table 4 Key resources and reagents for the SB system
From: In-house ELISA protocols for capsid p24 detection of diverse HIV isolates
Type | Reagent | Source | Identifier |
|---|---|---|---|
Antibodies | Mouse Anti-HIV-CA-p24 (capture) | Sino Biological | 11695-MM08 |
Mouse Anti-HIV-CA-p24 HRP conjugate (detection) | 11695-MM15 | ||
Dilution buffer | PBS tablets | Gibco | 18912-014 |
Tween 20 | Merck | 822184 | |
Protein standard | HIV Gag CA-p24 | Aalto Bio Reagents | AG 6054 |
Conjugate | HRP Stabilizer | Abcam | ab270548 |
Substrate solution | Lumi-Phos HRP | Lumigen | PSA-100 |
96-well plates | Half-area white 96-well plates | Greiner | 675074 |
Washer | BioTek ELx405 Select | Agilent | |
Shaker | Microplate shaker | VWR | 444-0270 |
Plate reader | GloMax® Navigator Microplate Luminometer | Promega | GM2010 |