Table 3 Key resources and reagents
From: In-house ELISA protocols for capsid p24 detection of diverse HIV isolates
Type | Reagent | Source | Identifier |
|---|---|---|---|
Antibodies | Anti-HIV Gag CA-p24 (capture) | Aalto Bio Reagents | D7320 |
HIV-CA-p24, Alkaline Phosphatase Conjugate monoclonal antibody (mAb) (detection) | BC1071-AP | ||
PBS tablets | Gibco | 18912-014 | |
Tween 20 | Merck | 822184 | |
Diluent solution, Blocking buffer | Empigen | Sigma-Aldrich | 45165 |
Skim Milk | SIAL | 70166 | |
Protein standard | HIV Gag CA-p24 | Aalto Bio Reagents | AG 6054 |
Substrate solution | LumiPhos Plus | Lumigen | P-7000 |
96-well plates | Half-area white 96-well plates | Greiner | 675074 |
Washer | BioTek ELx405 Select | Agilent | |
Shaker | Microplate shaker | VWR | 444-0270 |
Plate reader | GloMax® Navigator Microplate Luminometer | Promega | GM2010 |