Table 1 Primers used for whole genome amplification of PEDV CH/HLJ/18 using RT-PCR
Primer name | Sequence (5'–3') | Product size(bp) | ||
|---|---|---|---|---|
PEDV-1 | PEDV-1-F | AAGTGCTGTGCTGTCCTCTAG | 2032Â bp | |
PEDV-1-R | TCTGTAAAACCCGTCACTCTC | |||
PEDV-2 | PEDV-2-F | CTGCCTGTGACTGCTTAAAGG | 2323Â bp | |
PEDV-2-R | AACCATCAATAGCCATAGCAG | |||
PEDV-3 | PEDV-3-F | GTCTGTGGTTGTGGTACTGG | 1857Â bp | |
PEDV-3-R | GTGGACAAAGACCATCCTCG | |||
PEDV-4 | PEDV-4-F | GCTAGATTTAGGTTCAAGTCAG | 2307Â bp | |
PEDV-4-R | AACAGTAGGTATGGTAGTATGC | |||
PEDV-5 | PEDV-5-F | ACGTGTATGCGTGTAGGTGC | 2588Â bp | |
PEDV-5-R | GTGAAACATAACCACTTGAAGAT | |||
PEDV-6 | PEDV-6-F | TGGTAGCGACTTAGATGGTGTT | 2440Â bp | |
PEDV-6-R | GCAAAGTGTCAACAATGAAGAG | |||
PEDV-7 | PEDV-7-F | ATCCTGCTAAGACCTACA | 1949Â bp | |
PEDV-7-R | GCTGTGTCATAGTGGGCA | |||
PEDV-8 | PEDV-8-F | TAAGGATTTTGACTACTATAGGTATAATAGACCCACTG | 631Â bp | |
PEDV-8-R | AGCCAATTCATTGCACAACCTGAAAAAG | |||
PEDV-9 | PEDV-9-F | GGGATTACCCAAAGTGCG | 1968Â bp | |
PEDV-9-R | CACGAGCGCGCTGTGGTAT | |||
PEDV-10 | PEDV-10-F | GCTTAGTGCCCTATTACCAATTGATTGGTAAGC | 626Â bp | |
PEDV-10-R | AAAACACTGCTTGCTGTCAGGGTG | |||
PEDV-11 | PEDV-11-F | CTCGTGTTGAGTGTTATGATGG | 2479Â bp | |
PEDV-11-R | TGGGTAGTGATGTCTTGTTTGT | |||
PEDV-12 | PEDV-12-F | ACTCGCTGTAGGTCACCACT | 2421Â bp | |
PEDV-12-R | CTGCAGGCGCCTGAACATTA | |||
PEDV-S-1 | PEDV-S-1-F | ATGAAGTCTTTAACCTACTT | 1420Â bp | |
PEDV-S-1-R | CCTGAGAACACTTGAGTTGG | |||
PEDV-S-2 | PEDV-S-2-F | GCAGTAATTCCTCAGATCCTC | 1164Â bp | |
PEDV-S-2-R | GGTAGAAGAAACCAGGCAACT | |||
PEDV-S-3 | PEDV-S-3-F | GTCACAATTAATTTCACTGGTCATGG | 1325Â bp | |
PEDV-S-3-R | GGTAGCTAACTGTAGAGCCTCTTC | |||
PEDV-S-4 | PEDV-S-4-F | GATTCTGGACAGTTGTTAGC | 2184Â bp | |
PEDV-S-4-R | AGACTTTGAGACATCTTTGACA | |||
PEDV-ORF3 | PEDV-ORF3-F | ACACTAGTTGACCTTGAGTGGC | 1255Â bp | |
PEDV-ORF3-R | GAACGCAGAGTACTTGTAATGGC | |||
PEDV-E | PEDV-E-F | TATCTAGCTATACGTGGGCGGC | 617Â bp | |
PEDV-E-R | AAAGACCCAATTGACCTGAAAG | |||
PEDV-M | PEDV-M-F | AGTCTTACATGCGAATTGACC | 765Â bp | |
PEDV-M-R | AGCTGACAGAAGCCATAAAGT | |||
PEDV-MN | PEDV-MN-F | GTCAATAGCATTCGGTTGTGGCG | 841Â bp | |
PEDV-MN-R | TTTGCACGTGAAGTAGGAGGTG | |||
PEDV-N | PEDV-N-F | CCGAGTTCGGTTCTCACAGAT | 1415Â bp | |
PEDV-N-R | CATAGCCTGAGGCATCAACAC | |||
PEDV-5’UTR | PEDV-5’UTR-R | GATTACGCCAAGCTTCTGGCACGATGTTACCACCACGACGAC | 682 bp | |
PEDV-3’UTR | PEDV-3’UTR-F | GATTACGCCAAGCTTATTCCCAAGGGCGAAAATAGCGTAGC | 871 bp | |