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Fig. 1 | Virology Journal

Fig. 1

From: Porcine epidemic diarrhea virus strain CH/HLJ/18 isolated in China: characterization and phylogenetic analysis

Fig. 1

Isolation and cultivation of PEDV in Vero cells. (a) Identification of PEDV and absence of TGEV/PDCoV in small intestinal tissue sample. Lane 1: Small intestine tissue sample of diarrhea piglets; Lane 2: small intestine tissue sample of piglets infected with PEDV/TGEV/PDCoV as a positive control; Lane 3: non-template control (NTC). (b) Appearance of PEDV-infected and non-infected Vero cells in M199. (c) Identification of PEDV in Vero cells during serial passages using RT-PCR. Lanes 1–5: blind transmission of PEDV cell culture from the 1st to 5th generation; Lane 6: NTC. (d) Plaque morphology of PEDV at different dilutions using the direct agarose overlay plaque assay

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Virology Journal

ISSN: 1743-422X