Fig. 6

Identification of SKAI-DS84 binding mechanism. Structure of a SKAI-DS84 antibody and b RBD using in silico rosetta antibody c binding modeling of SKAI-DS84 antibody with B.1.617.2 and B.1.1.529 RBD using Cluspro2. d In total, 176,930 SARS-CoV-2 RBD residues consisting of 15 to 20 amino acid peptides were prepared on a chip and incubated with 1 ng/mL SKAI-DS84 to confirm antigen–antibody binding. The interaction (green) or lack of it (black) between RBD and SKAI-DS84 is shown by dots on the panels. e, f Quaternary epitope presentation by the SARS-CoV-2 RBD was assessed by testing for binding by representative quaternary epitope antibodies. Fluorescence-based dot blot analysis of quaternary SKAI-DS84 binding to either S or RBD proteins. SARS-CoV-2, severe acute respiratory syndrome coronavirus 2; RBD, receptor-binding domain