Fig. 3

Phylogenetic tree was constructed using the Neighbor-Joining method with the bootstrap test (1000 replicates) using MEGA 11 software. The evolutionary distances were computed using the p-distance method and are in the units of the number of base differences per site. (A) Phylogenetic analysis of G gene sequences of RSV-A strains circulating in Shanghai, September 2017–December 2021. The present study RSVA strains are indicated by ‘‘red circles’’ followed by their NCBI accession numbers. RSV-A reference strains representing known genotypes were retrieved from GenBank and included in the tree (labels include accession number). Human metapneumovirus (MK588637) was used as an outgroup. The scale bar represents the number of nucleotides substitutions per site. (B) Phylogenetic analysis of G gene sequences of RSV-B strains circulating in Shanghai, September 2017–December 2021. The present study RSV-B strains are indicated by ‘‘red circles’’ followed by their NCBI accession numbers. RSV-B reference strains representing known genotypes were retrieved from GenBank and included in the tree (labels include accession number). Human metapneumovirus (MK588637) was used as an outgroup. The scale bar represents the number of nucleotides substitutions per site