Fig. 1From: Hypovirulence-associated mycovirus epidemics cause pathogenicity degeneration of Beauveria bassiana in the fieldDetection and infection proportion of BbCV2 virus in B. bassiana strains, and analysis of genetic diversity from different collection sites. A dsRNA mycovirus detection of B. bassiana strains. Left: dsRNA extraction of all tested fungal strains; (+) = dsRNA from strains without DNase I and S1 nuclease treatment; (−) = dsRNA from strains with DNase I and S1 nuclease treatment. Right: detection of BbCV2 infection in B. bassiana strains by RT-PCR; (+) = original virus-infected B. bassiana strain of BbOFZK152 as positive control; (−) = original virus-free B. bassiana strain of BbOFDH-bar as negative control. B Proportion of BbCV2 infection in B. bassiana strains from different collection sites. C Nucleotide phylogenetic tree of virus BbCV2 collected from different regions and 32 mycovirusesBack to article page