From: CRISPR–Cas system to discover host-virus interactions in Flaviviridae
Virus | CRISPR screen | Cell type | CRISPR target genes | References |
---|---|---|---|---|
ZIKV | CRISPRa | Huh7 | TMEM120A | [77] |
Huh7.5 | IFI6, IFN-λ2 | [79] | ||
CRISPR KO | Human pluripotent stem cell (hPSC)-derived neural progenitors (NPs) | ISG15, SOCS3, STAT3 | [80] | |
A549 | RIG-I, MDA5, IFNAR | [82] | ||
A549 | OAS3, RNAseL, STAT1, STAT2, MAVS | [85] | ||
DENV | CRISPR KO | A549 | OAS3, RNAseL, STAT1, STAT2, MAVS | [85] |
WNV | CRISPR KO | A549 | OAS1, OAS2, OAS3, RNAse L | [83] |
YFV and other flaviviruses | CRISPR KO | Huh7.5 | IFI6, HSPA5 | [49] |
HCV | CRISPR KO | Huh7.5 | STAT1, STAT2, IRF9, STAT3, STAT6 | [84] |