From: CRISPR–Cas system to discover host-virus interactions in Flaviviridae
Type of genetic screen | Virus | Cell line | Top screening hits | References |
---|---|---|---|---|
CRISPR KO | DENV | Huh7.5.1 | OST complex (STT3A and STT3B), TRAP complex (SSR1, SSR2, SSR3), ERAD (SEL1L, AUP1, DERL2) | [14] |
Huh7.5.1 | MAGT1 | [50] | ||
Huh7.5.1, HEK293FT | EMC | [51] | ||
HAP1 | DPM1 and DPM3 | [52] | ||
HAP1 | RACK1 | [53] | ||
ZIKV | H1-HeLa | EMC, AXL, STT3A, TRAP complex | [54] | |
HAP1 | TMEM41B and VMP1 | [55] | ||
Huh7.5 | RACK1 | [56] | ||
WNV | 293FT | EMC2, EMC3, SEL1L, DERL2, UBE2G2, UBE2J1, HRD1 | [57] | |
293T | STTA3, SPCS1, SPCS3, SEC63 | [58] | ||
YFV | HAP1 | TMEM41B and VMP1 | [55] | |
HCV | Huh7.5 | CD81, OCLN, CLDN1 | ||
Huh7.5.1 | ELAVL1, RFK, FLAD1 | [14] | ||
CRISPRi | HCV | Huh7.5 | TRIM26 | [61] |
CRISPRa | ZIKV | A549,SNB-19 | RhoV, WWTR1 | [62] |