Fig. 6

Aspirin promotes the expression of IRF-1, COX2 and PGE2

Caco-2, CV-1 and MA104 cells were infected with rotavirus (10⁷ FFU/ml) and 0.6, 0.3, 0.15, and 0.08 mol/ml of aspirin were added for 12 h. qRT-PCR was performed to analyses IRF-1 mRNA expression in each of the three host cells (A). Data are presented as the average ± SD. ***P < 0.0001. (B) The levels of COX-2 mRNA expression were determined by qRT-PCR using COX-2-specific primers in infected cells relative to noninfected cells at 12 h. Error bars indicate the standard errors of the means (n = 3). (C) Secreted PGE2 in the media was quantified using the ELISA method. Significant differences (*, P 0.01;) between samples are indicated. Error bars indicate the standard errors of the means (n = 3)