Fig. 2

Immunofluorescent assay of the antiviral activity of aspirin against rotavirus

Fluorescent focus assays were performed for quantification of the antiviral activity against rotavirus in different host cells. (A), Caco-2 (B), CV-1 (C) MA104. Different concentrations of aspirin were added in the culture medium after virus infection (10⁷ FFU/ml). After 12 h, supernatants were collected, and virus titers were determined by the immunofluorescence method. Non-treated virus-infected cells were used as a negative control, and ribavirin (0.2 mol/ml) as an internal positive control. Virus titer= (Fluorescence quantity × Dilution ratio)/volume of virus added per well × 1000. RV, rotavirus; ASP, aspirin. *P = 0.0003, **P = 0.0002, ***P = 0.0001