Fig. 1

qRT-PCR assay of the dose-dependent antiviral activity of aspirin against rotavirus

Cells were infected with the rotavirus-SA11 strain, and then different concentrations of aspirin (0.6, 0.3, 0.15, and 0.08 mol/ml) were added to the culture medium for 12 h. Levels of expression of the rotavirus VP6 gene were determined by qRT-PCR assay. The percentage inhibition in the cell cultures (relative to infected but non-treated cells) is shown for MA104 (A), Caco-2 (B), and CV-1 (C) cells. Ribavirin (0.2 mol/ml) was included as an internal positive control. Data were derived from three replicates and are representative of three independent experiments. All viruses were used with 107 FFU/ml. RV, rotavirus; ASP, aspirin. * p < 0.05; ** p < 0.01; *** p < 0.001