Investigating the interactions of endornaviruses with each other and with other viruses in common bean, Phaseolus vulgaris

Table 5 The effects of Phaseolus vulgaris endornavirus (PvEV) 1, PvEV2 and PvEV3 on the accumulation of cucumber mosaic virus RNA accumulation

Endornavirus Content	Plant Line	Mean relative CMV RNA accumulation ± SEM*	Group
No endornavirus	GLP24	9.95 ± 5.11	2
	KATX56	3.19 ± 1.37	1
	Wairimu	21.48 ± 13.19	3
PvEV1	RED40	10.48 ± 3.03	2
	RWR1668	32.42 ± 9.90	3
	GLP1127	21.67 ± 7.88	3
PvEV1 & PvEV2	KK022	3.60 ± 2.75	1
	SER16	2.23 ± 0.89	1
	RWR2245	30.50 ± 10.72	3
PvEV1, PvEV2 & PvEV3	KK072	8.63 ± 4.52	2
	RWR2075	17.16 ± 7.93	3
	MCM2001	3.18 ± 2.60	1

*Reverse transcription-quantitative polymerase chain reaction assays with appropriate primers (Table S2) were used to measure endornavirus RNA accumulation in twelve cultivars of Phaseolus vulgaris (Table S1), relative to accumulation of two housekeeping gene transcripts, PvActin 11 and PvUnknown 1 [29]. Plants were infected with cucumber mosaic virus (CMV). Plant lines were assigned to groups of low (1), medium (2) or high (3) CMV RNA accumulation based on inspection of mean values and p-values but were not statistically significantly different at a 95% confidence interval. RNA was isolated from 10 individual plants of each line to assay for each endornavirus (i.e., n = 10 plants per line per endornavirus) with the following exceptions: KATX56 and KK022 (n = 11). Wairimu = Wairimu Dwarf

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