Investigating the interactions of endornaviruses with each other and with other viruses in common bean, Phaseolus vulgaris

Table 2 The effects of cucumber mosaic virus infection on the accumulation of endornavirus RNAs

Plant Line	Mean relative endornavirus RNA accumulation ± SEM*
	PvEV1		PvEV2		PvEV3
	Mock	CMV	Mock	CMV	Mock	CMV
RED40	1.20 ± 0.23	4.51 ± 1.39	n/a	n/a	n/a	n/a
RWR1668	1.31 ± 0.30	0.88 ± 0.24	n/a	n/a	n/a	n/a
GLP1127	1.37 ± 0.28	1.33 ± 0.51	n/a	n/a	n/a	n/a
KK022	1.91 ± 0.89^a	0.25 ± 0.056^b	1.77 ± 0.67^a	0.32 ± 0.18^b	n/a	n/a
SER16	1.11 ± 0.20^a	0.43 ± 0.20^b	1.93 ± 0.88	4.17 ± 2.67	n/a	n/a
RWR2245	1.22 ± 0.26^a	5.98 ± 2.97^b	1.15 ± 0.20	7.31 ± 3.43	n/a	n/a
KK072	1.50 ± 0.55	5.28 ± 1.50	1.18 ± 0.29	9.48 ± 3.86	1.56 ± 0.59	6.37 ± 1.73
RWR2075	1.76 ± 0.61	2.70 ± 0.53	1.11 ± 0.17	1.16 ± 0.16	1.06 ± 0.13	1.33 ± 0.33
MCM2001	1.65 ± 0.52^a	0.11 ± 0.026^b	1.17 ± 0.22^a	0.11 ± 0.012^b	2.48 ± 0.58^a	0.28 ± 0.062^b

^*Reverse transcription-quantitative polymerase chain reaction assays with appropriate primers (Table S2) were used to measure endornavirus RNA accumulation in nine cultivars of Phaseolus vulgaris (Table S1), relative to accumulation of two housekeeping gene transcripts, PvActin 11 and PvUnknown 1 [29]. Plants were either infected with cucumber mosaic virus (CMV) or mock inoculated. Statistically significant differences (p < 0.05) in PvEV1, 2 and 3 RNA accumulation between mock-inoculated and infected with CMV, as determined by Wilcoxon Rank sum test, are indicated by different lower-case letters. RNA samples were isolated from 10 individual plants of each line to assay for each endornavirus (i.e., n = 10 plants per line per endornavirus) with the following exceptions: RED40 CMV infected (n = 11); GLP1127 mock-inoculated (n = 11); KK022 mock-inoculated (n = 9); KK022 CMV infected (n = 11); SER16 mock-inoculated (n = 7); RWR2245 mock-inoculated (n = 9); KK072 mock-inoculated (n = 7). ‘n/a’ indicates not assayed, i.e., plant line does not contain the indicated endornavirus

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