Investigating the interactions of endornaviruses with each other and with other viruses in common bean, Phaseolus vulgaris

Table 1 The relative steady-state RNA accumulation for the endornaviruses PvEV1, PvEV2 and PvEV3 in nine P. vulgaris cultivars

Plant Line	Mean relative endornavirus RNA accumulation ± SEM*
Plant Line	PvEV1	PvEV2	PvEV3
RED40	4.28 ± 0.96	n/a	n/a
RWR1668	5.01 ± 1.31	n/a	n/a
GLP1127	2.73 ± 0.96	n/a	n/a
KK022	4.13 ± 1.34	0.40 ± 0.15^a	n/a
SER16	7.38 ± 2.78	0.0052 ± 0.0035^b	n/a
RWR2245	4.54 ± 1.57	0.32 ± 0.12^a	n/a
KK072	2.43 ± 1.26	0.049 ± 0.016^c	0.028 ± 0.0086
RWR2075	7.08 ± 2.29	0.064 ± 0.016^c	0.024 ± 0.0062
MCM2001	8.97 ± 2.39	0.0018 ± 0.00054^b	0.013 ± 0.0026

^* Reverse transcription-quantitative polymerase chain reaction assays with appropriate primers (Additional file 1: Table S2) were used to measure endornavirus RNA accumulation in nine cultivars of Phaseolus vulgaris (Additional file 1: Table S1), relative to accumulation of two housekeeping gene transcripts, PvActin 11 and PvUnknown 1 [29]. There were no statistically significant differences (p < 0.05) in relative RNA steady-state accumulation across the cultivars for PvEV1 as determined by Kruskal–Wallis (χ² = 9.2722, df = 8, p = 0.3199, n = 90), or for PvEV3 (χ² = 2.0004, df = 2, p = 0.3678, n = 30). Statistically significant differences (p < 0.05) in PvEV2 RNA accumulation as determined by Kruskal–Wallis (χ² = 45.511, df = 5, p = 1.142e-08, n = 60) are indicated by different lower-case letters. Pairwise comparisons were performed using a Wilcoxon rank sum test with Benjamini–Hochberg p-value correction. RNA samples were isolated from 10 individual plants of each line to assay for each endornavirus (i.e., n = 10 plants per line per endornavirus). ‘n/a’ indicates not assayed, i.e., plant line does not contain the indicated endornavirus

ISSN: 1743-422X