Table 1 Primer sequence
Genes | Orientation | Primer sequence (5’-3’) |
|---|---|---|
SIRT1 | Forward | TAGCCTTGTCAGATAAGGAAGGA |
Reverse | ACAGCTTCACAGTCAACTTTGT | |
GAPDH | Forward | CTCGCTTCGGCAGCACA |
Reverse | AACGCTTCACGAATTTGCGT | |
miR-194-3p | Forward | ACACTCCAGCTGGGCCAGTGGGGCTGCTGT |
Reverse | TGGTGTCGTGGAGTCG | |
U6 | Forward | CTCGCTTCGGCAGCACA |
Reverse | AACGCTTCACGAATTTGCGT | |
VP6 | Forward | CAGTGATTCTCAGGCCGAATA |
Reverse | GGCGAGTACAGACTCACAAA | |
Beclin1 | Forward | CTGAGGGATGGAAGGGTCTAAGA |
Reverse | CACGGTCCAGGATCTTGAAACTC |