Fig. 7

MiR-194-3p depresses p53 acetylation by modulating SIRT1 during RV infection. (A) Caco-2 cells were infected with RV-Wa (MOI = 1) or DMEM (Mock) for12 hpi, followed by western blot with anti-p53 and anti-Ac-p53 antibody. (B) Caco-2 cells transfected with miR-194-3p mimics or inhibitors or sh-NC, sh-SIRT1#1 or sh-SIRT1#2 or miR-194-3p mimics + oe-NC or miR-194-3p mimics + oe-SIRT1 were infected with RV-Wa (MOI = 1) for 12 hpi, the cells were then harvested for detection. Western blot were used to detect the expression of SIRT1, p53 and acetylation of p53 in each group of cells. The fold change was calculated after normalized with GAPDH. The data are presented as the mean ± SD. * vs. the miR-NC, P < 0.05. + vs. the in-NC, P < 0.05. # vs. the sh-NC, P < 0.05; ^ vs. the miR-194-3p + oe-NC, P < 0.05