Fig. 6

Upregulating miR-194-3p or depressing SIRT1 restrained RV replication in vivo. (A) The suckling mice were respectively treated with 10 nmol agomir-NC, miR-194-3p agomir, DMEM, and EX527 (3.15 mg/kg) by oral gavages and then inoculated with 30 µL RV through oral gavages, and the intestinal tissue were harvested for detection at 96 hpi. The expression of miR-194-3p, VP6 and SIRT1 were detected by qRT-PCR. (B) The protein expression of SIRT1 in suckling mice were detected by western blot. (C) The mean diarrheal score for each group at days form 12–96 hpi. D/E. The expression of VP6 in intestinal tissue was determined by immunofluorescence analysis and western blot, respectively. F. The lipid droplet formation in intestinal tissue was detected by Oil red O staining. The fold change was calculated after normalized with GAPDH. The data are presented as the mean ± SD. a vs. the Normal, P < 0.05. b vs. the agomir-NC, P < 0.05. c vs. the DMEM, P < 0.05