Fig. 3

MiR-194-3p regulates RV replication by targeting SIRT1. (A) Bioinformatics website RNA22 was used to detect the binding site of SIRT1 and miR-194-3p. (B) Caco-2 cells transfected with miR-194-3p mimics or NC oligonucleotides were infected with RV-Wa (MOI = 1) or DMEM (Mock) for 24 h. The interaction between miR-194-3p and SIRT1 was verified by dual-luciferase reporter assay. (C) Caco-2 cells were infected with RV-Wa at an MOI of 1 for 24 h, the interaction between miR-194-3p and SIRT1 was detected by RIP analysis. D/E. Caco-2 cells were infected with RV-Wa at an MOI of 1, the culture supernatant and cell lysates were collected at indicated time points (0, 12, 24, 36, 48 hpi), the mRNA and proteins expression of SIRT1 and VP6 were determined by qRT-PCR and western blot, respectively. F/G. Caco-2 cells transfected with NC oligonucleotides, miR-194-3p mimics or inhibitors were infected with RV-Wa (MOI = 1) for 24 h, the mRNA and proteins expression of SIRT1 and VP6 were determined by qRT-PCR and western blot, respectively. The fold change was calculated after normalized with GAPDH. The data are presented as the mean ± SD. * vs. the miR-NC, P < 0.05; + vs. the in-NC, P < 0.05