Fig. 2

MiR-194-3p represses RV replication and autophagy during RV infection. (A) Caco-2 cells were transfected with negative control (NC) oligonucleotides, miR-194-3p mimics or inhibitors for 36 h, and the expression of miR-194-3p was determined by qRT-PCR. The fold change was calculated after normalized with U6. (B) Caco-2 cells transfected with NC oligonucleotides, miR-194-3p mimics or inhibitors were infected with RV-Wa (MOI = 1) for 24 h, the expressions of viral structural proteins VP6 and VP7 were determined by western blot. (C) The expressions of VP6 was determined by Immunofluorescence analysis. (D) The antiviral activity were determined by using CPE inhibition assays. (E) The infectious virus titers in cell supernatants were evaluated by using plaque assays. F/G. The survival rate of Caco-2 cells were assessed by MTT and plate cloning assays. H. The expressions of LC3 was determined by Immunofluorescence analysis. I. The expressions of autophagy-linked protein Beclin1 was detected by western blot. J. Caco-2 cells transfected with NC oligonucleotides, miR-194-3p mimics or miR-NC were infected with RV-Wa (MOI = 1), and then treated with rapamycin (100 nM) or bafilomycin A1 (Baf-A1, 100 nM) for 24 h, the cells were then harvested for detection. The expression of Beclin1 and LC3 were determined by western blot. The fold change was calculated after normalized with GAPDH. The data are presented as the mean ± SD. * vs. the miR-NC, P < 0.05; + vs. the in-NC, P < 0.05