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Fig. 4 | Virology Journal

Fig. 4

From: Antibody production and characterization of the nucleoprotein of sever fever with thrombocytopenia syndrome virus (SFTSV) for effective diagnosis of SFTSV

Fig. 4

Expression and purification of truncated SFTSV N protein. A Scheme of SFTSV N truncation. B SDS-PAGE (top) and western blot (bottom) analyses of SFTSV truncates. Anti-his-tag antibody was used as primary antibody in western blot. Lane M—molecular weight marker; Lane 1—full-length SFTSV N protein (1–245); Lanes 2–6—Truncated SFTSV N proteins, dN1 (13–245), dN2 (33–245), dN3 (47–245), dN4 (65–245), and dN5 (75–245), respectively; dN2 and dN3 were lysed and purified in 0.8 M urea

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Virology Journal

ISSN: 1743-422X