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Fig. 1 | Virology Journal

Fig. 1

From: Antibody production and characterization of the nucleoprotein of sever fever with thrombocytopenia syndrome virus (SFTSV) for effective diagnosis of SFTSV

Fig. 1

Expression and purification of SFTSV N protein. A Western blot analyses of full-length denatured SFTSV N protein. Anti-his-tag antibody was used as the primary antibody. Lane M—molecular size marker; Lane 1—His-GFP as the positive control for His antibody; Lane 2—expressed SFTSV N protein. B SDS-PAGE analysis of purified SFTSV N protein. Lane M = molecular size marker; Lane 1 = non-bound (flow through); Lane 2 = washed with binding buffer (100 mM Tris, 200 mM NaCl, 10 mM imidazole, pH8.0), Lanes 3–6: Purified SFTSV N protein eluted with 50 mM, 100 mM, 150 mM, or 200 mM imidazole, respectively. C Comparison of native and denatured SFTSV N protein after separation by SDS-PAGE or native-PAGE. The native-PAGE results indicate that the native SFTSV N protein was oligomerized

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Virology Journal

ISSN: 1743-422X