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Fig. 5 | Virology Journal

Fig. 5

From: Dengue virus 3 genotype I (GI) lineage 1 (L1) isolates elicit differential cytopathic effect with syncytium formation in human glioblastoma cells (U251)

Fig. 5

Different CPE patterns of DENV3 GIL1 infection in mosquito cells. C6/36 cells were infected with MOI 0.1 of DENV3 MG-20 or DENV3 PV_BV, and photos were taken at 0 and 6 DPI, respectively, as shown in panels 5A-B for cells infected with DENV3 MG-20 (arrow indicates syncytia formation) and 5D-E for cells infected with DENV3 MG-20. 5C and 5F. Infected C6/36 cells with DENV3 MG-20 and DENV3 PV_BR, respectively, diluted to 10− 4 and overlayed with CMC were stained at 6 DPI with crystal violet. 5G-I. For immunofluorescence, cells were infected and at 6 DPI treated Alexa fluor 488 as secondary antibody for identification of dengue envelope/4G2 monoclonal antibody complex (green color), and with DAPI to identify the nucleus (blue color). 5G Negative staining control. 5H DENV3 MG-20 cell infection with syncytium formation indicated by an arrow. 5I DENV3 PV_BR cell infection

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Virology Journal

ISSN: 1743-422X