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Fig. 3 | Virology Journal

Fig. 3

From: Dengue virus 3 genotype I (GI) lineage 1 (L1) isolates elicit differential cytopathic effect with syncytium formation in human glioblastoma cells (U251)

Fig. 3

Different patterns of CPE observed in glioblastoma cells (U251) infected with DENV3 GIL1 isolates. U251 cells were infected with MOI 0.1 of DENV3 MG-20 or DENV3 PV_BV, and photos were taken at 0, 3, and 6 DPI, respectively, as shown in panels 3 A-C for cells infected with DENV3 MG-20 and 3D-F for cells infected with DENV3 PV_BV. Photos from optical microscopy were taken under 100x magnification. 3G-L: For immunofluorescence, cells were infected and at 4 DPI treated Alexa fluor 488 as secondary antibody for identification of dengue envelope/4G2 monoclonal antibody complex (green color), and with DAPI to identify the nucleus (blue color). 3G-I. Cells infected with DENV3 MG-20. 3J-L. Cells infected with DENV3 PV_BR. The immunofluorescence photos were taken under 200x magnification. Arrow indicates syncytium formation

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Virology Journal

ISSN: 1743-422X