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Fig. 7 | Virology Journal

Fig. 7

From: DENV-2 NS1 promotes AMPK-LKB1 interaction to activate AMPK/ERK/mTOR signaling pathway to induce autophagy

Fig. 7

NS1 acts as an assembly platform for LKB1-AMPK interactions and promotes LKB1-AMPK interactions. A NS1 interacts with LKB1. B NS1-Wing domain interacts with LKB1. C NS1 interacts directly with LKB1. D NS1-Wing domain has a direct interaction with LKB1. E Direct interaction between LKB1 and NS1. F Co-localization of NS1 with LKB1 exists in HUVEC cells overexpressing NS1. Red fluorescence indicates DENV-2 NS1, green fluorescence indicates LKB1, and blue fluorescence indicates nuclei. Scale bar: 25 μm. G NS1 promotes the interaction of AMPKα1 with LKB1. Glutathione gel beads loaded with GST protein and GST-LKB1 protein were co-incubated with in vitro-expressed HA-tagged DENV-2 NS1 and AMPKα1 in the presence and absence of DENV-2 NS1 addition, respectively. H Effect of NS1 on the interaction of AMPKα2 with LKB1. Glutathione gel beads loaded with GST protein and GST-LKB1 protein were co-incubated with in vitro-expressed HA-tagged DENV-2 NS1 and AMPKα2, respectively, with and without DENV-2 NS1 addition

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Virology Journal

ISSN: 1743-422X