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Fig. 5 | Virology Journal

Fig. 5

From: DENV-2 NS1 promotes AMPK-LKB1 interaction to activate AMPK/ERK/mTOR signaling pathway to induce autophagy

Fig. 5

Interaction of DENV NS1 with the S, C, and U structural domains of AMPKa. A The fusion proteins DENV-2 NS1 and GST-only with GST tag and AMPKα1 with HA tag were subjected to GST pulldown assay in vitro, respectively. B The fusion proteins DENV-2 NS1 and GST-only with GST tag and AMPKα2 with HA tag were subjected to GST pulldown assay in vitro, respectively. C AMPKα1 was divided into three structural domains by STC, C-terminal, and UBA-like, and glutathione gel beads loaded with GST protein and GST-AMPKα1S, GST-AMPKα1C, and GST-AMPKα1U proteins were co-incubated with HA-tagged NS1 expressed in vitro, respectively. D AMPKα2 was divided into STC, C-terminal, and UBA-like structural domains by structural domain, and glutathione gel beads loaded with GST protein and GST-AMPKα2S, GST-AMPKα2C, and GST-AMPKα2U proteins were co-incubated with HA-tagged NS1 expressed in vitro, respectively

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Virology Journal

ISSN: 1743-422X