Table 1 Sequences of oligonucleotides used in this study
Primer | Sequence | Details |
|---|---|---|
gD-F | TCTCGAGGAGGACCCGTGCGGGGTGGTGGCG | Left Homologous arm |
gI-R | TGAATTCGCAGGCGCGCTTGGGGTCGAGGCGC | |
US2-F | TACTAGTGCTGGACACGGAGTGGTCGTCCGTCC | Right Homologous arm |
US2-R | TGCGGCCGCGTGAGGCGGGCCGCGCCCCGCTCT | |
sgRNA-gI-F | CACCGTACGACCCCGCGTCCCCCG | sgRNA-gI |
sgRNA-gI-R | AAACCGGGGGACGCGGGGTCGTAC | |
sgRNA-US2-F | CACCGGGGGTGACGGCCATCACCG | sgRNA-US2 |
sgRNA-US2-R | AAACCGGTGATGGCCGTCACCCCC | |
gI/US2-F | ACCACCGCCGCGCCGGGCGTCTCGCGCCAC | Identify the deleted genes |
gI/US2-R | GGCCAGCGAGCCGGGGGAGATCTCCGAGGA |