Table 1 Primer sequences for quantitative real-time PCR
Target | Purpose | Prime name | Sequences (5’ to 3’) |
|---|---|---|---|
Segment 1 mRNA | Reverse transcription | PR8 seg1 mRNA tag | CCAGATCGTTCGAGTCGTTTTTTTTTTTTTTTTTAAACTATTCGA |
Realtime PCR | mRNAtag | CCAGATCGTTCGAGTCGT | |
PR8 seg1 mRNA Re | GGAGATATGGGCCAGCATTA | ||
Segment 4 mRNA | Reverse transcription | PR8 seg4 mRNA tag | CCAGATCGTTCGAGTCGTTTTTTTTTTTTTTTTTCCTCATATTTCT |
Realtime PCR | mRNAtag | CCAGATCGTTCGAGTCGT | |
PR8 seg4 mRNA Re | GGGCAATCAGTTTCTGGATGTGTTCT | ||
Segment 5 mRNA | Reverse transcription | PR8 seg5 mRNA tag | CCAGATCGTTCGAGTCGTTTTTTTTTTTTTTTTTCTTTAATTGTC |
Realtime PCR | mRNAtag | CCAGATCGTTCGAGTCGT | |
PR8 seg5 mRNA Re | CGATCGTGCCTTCCTTTG | ||
Target | Purpose | Prime name | Sequences (5’ to 3’) |
Segment 1 mRNA | Reverse transcription | PR8 seg1 mRNA tag | CCAGATCGTTCGAGTCGTTTTTTTTTTTTTTTTTAAACTATTCGA |
Realtime PCR | mRNAtag | CCAGATCGTTCGAGTCGT | |
PR8 seg1 mRNA Re | GGAGATATGGGCCAGCATTA | ||
Segment 4 mRNA | Reverse transcription | PR8 seg4 mRNA tag | CCAGATCGTTCGAGTCGTTTTTTTTTTTTTTTTTCCTCATATTTCT |
Realtime PCR | mRNAtag | CCAGATCGTTCGAGTCGT | |
PR8 seg4 mRNA Re | GGGCAATCAGTTTCTGGATGTGTTCT | ||
Segment 5 mRNA | Reverse transcription | PR8 seg5 mRNA tag | CCAGATCGTTCGAGTCGTTTTTTTTTTTTTTTTTCTTTAATTGTC |
Realtime PCR | mRNAtag | CCAGATCGTTCGAGTCGT | |
PR8 seg5 mRNA Re | CGATCGTGCCTTCCTTTG |