Fig. 2
From: Replication of cowpox virus in macrophages is dependent on the host range factor p28/N1R
CPXV-∆p28, CPXV-p28(1-184) and CPXV-p28∆RING replication kinetics. The replication of CPXV in the Rat-2 fibroblast cells and J774A.1 mouse macrophages was analysed via qPCR and plaque assay (n = 2). Shown are ∆cT values as a measure of viral DNA replication in CPXV-infected (MoI = 0.1) a Rat-2 and c J774A.1 cells or infectious particles (pfu/ml) in the supernatant of b Rat-2 or d J774A.1 cells infected with the p28 mutant viruses CPXV-∆p28, CPXV-p28(1-184) and CPXV-p28∆RING. ∆cT values were obtained via normalization to expression of the cellular MYC gene. Statistics: two-way ANOVA and Tukey’s multiple comparison test. Asterisks showing significance of CPXVΔp28 vs. WT/CPXV p28(1-184) vs. WT/CPXVp28ΔRING vs. WT)