Fig. 3From: One-step real-time multiplex reverse transcription-polymerase chain reaction assay with melt curve analysis for detection of potato leafroll virus, potato virus S, potato virus X, and potato virus YConfirmation of detection sensitivity for each potato virus. Ten-fold dilution of leaf homogenates was prepared by mixing virus-free and virus-infected potato leaf homogenates with a PLRV (isolate: ChLR_2), b PVSO (M), c PVX (O-IC249), or d PVYNTN (Eu-12Jp). The peaks (melting temperature) for plant EF1α and each virus are shown. Melt curve colors: black, virus-free potato leaf; red, a tenfold dilution of each virus; green, 100-fold of each; blue, 1000-foldBack to article page