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Fig. 1 | Virology Journal

Fig. 1

From: One-step real-time multiplex reverse transcription-polymerase chain reaction assay with melt curve analysis for detection of potato leafroll virus, potato virus S, potato virus X, and potato virus Y

Fig. 1

Detection of four potato viruses from total RNA extracted by simple RNA preparation. RNA solutions were prepared from virus-infected potato (cv. Irish Cobbler) leaf homogenate with a PLRV (isolate: ChLR_2), b PVSO (M), c PVX (O-IC249), or d PVYNTN (Eu-12Jp). Simultaneous detection of the four viruses was confirmed using e RNA solution prepared from the mixture of equal volume of four singly-virus-infetcted leaf homogenates. RNA solution prepared from f virus-free potato leaf homogenate (Healthy) was used as negative control. One-step real-time mRT-PCR was performed as described in Materials and Methods, and results analyzed by the instrument software; peaks (melting temperature) for plant EF1α and each virus are shown. The Ct value corresponding to each melt curve is given after the panel caption

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Virology Journal

ISSN: 1743-422X