Combination therapy of zinc and trimethoprim inhibits infection of influenza A virus in chick embryo

Table 1 It shows study groups

Group	Virus load	Treatment	Number of embryos
group 1	10 EID₅₀	Tri-Z: at Zn/TMP ratio 0.01 to 0.3, increment of 0.01 (30 ratios)	12 per ratio
group 2	100 EID₅₀	Tri-Z: at Zn/TMP ratio 0.01 to 0.3, increment of 0.01 (30 ratios)	12 per ratio
C 1	10 EID₅₀	.5 ml NS	12
C 2	10 EID₅₀	Zn: 0.05 to 1.5 mg with 0.05 mg increments (30 concentrations)	12 per concentration
C 3	10 EID₅₀	TMP: 5 mg	12
C 4	10 EID₅₀	0.2 ml of convalescent serum	12

We carried experiments examining effect of a mixture of trimethoprim and zinc influenza virus. We examined two virus loads 10 EID₅₀ and 100 EID₅₀, in 6 day old chick embryos, group 1 and group 2. This was compared to control groups (C 1- C 4). Experiments were repeated in triplicates. EID₅₀ = egg infective dose 50 i.e. virus load that cause 50% mortality of the embryo, C = control, NS = normal saline, TMP = trimethoprim, Zn = zinc, Tri-Z: mixture of trimethoprim and zinc oxide

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