Fig. 5
From: Resource-efficient internally controlled in-house real-time PCR detection of SARS-CoV-2
RKI/ZBS1 SARS-CoV-2 protocol performance with different PCR master mixes. In total 7 PCR mixes were compared with the RKI/ZBS1 SARS-CoV-2 protocol on a Bio-Rad CFX96 cycler. 10 clinical specimens of different RNA load and negative as well as positive controls were set up. Means of duplicate CT values are plotted against the respective master mix. Positive controls (crosses) are shown only for the SARS-CoV-2 assays E-Gene and orf1ab; negative controls are not shown