Table 3 Chemical inhibitors used in this study
From: Caveolin-1 is involved in encephalomyocarditis virus replication in BHK-21 cells
Chemical inhibitor | Effect | Concentration |
|---|---|---|
NH4Cl | Inhibits pH-dependent activation of the fusion protein | 10Â mM/20Â mM |
Bafilomycin A1 | Vacuolar-type ATPase inhibitor | 10Â nM/20Â nM |
Chlorpromazine | Inhibits clathrin-dependent endocytosis | 5 μM/10 μM |
Pitstop-2 | Cell-permeable clathrin inhibitor | 5 μM/10 μM |
Nystatin | Disrupts caveolae | 12.5 μg/25 μg |
MβCD | Extracts cholesterol from lipid membranes | 2.5 mM/5 mM |
Dynasore | Inhibits GTPase activity | 20 μM/40 μM |
Mitmab | Blocks the lipid binding | 5 μM/10 μM |
Cytochalasin D | Disrupts actin polymerization | 5 μM/10 μM |
Jasplakinolide | Stabilizes actin microfilaments | 5 μM/10 μM |
EIPA | NA+/H+ exchanger inhibitor | 10 μM/20 μM |
IPA-3 | the Pak-1 inhibitor | 7.5 μM/15 μM |
Wortmannin | the PI3K inhibitor | 5 μM/10 μM |