Fig. 4
From: Cohesin promotes HSV-1 lytic transcription by facilitating the binding of RNA Pol II on viral genes
Effects of Cohesin components depletion on HSV-1 the immediate-early and early gene expression. a BJ cells were infected with HSV-1 at an MOI 5 after SMC1 or Rad21 knockdown by shRNA. ICP0, ICP4, and ICP8 gene transcripts were detected by RT-qPCR at 6 h post-infection (hpi). Shown is mRNA fold-change over control at 0 hpi. Error bars represent the standard deviation from three independent experiments. b HeLa cells were transfected with control siRNA or siRNA targeting SMC1 or Rad21 for 48 h. These cells were then infected with HSV-1 at an MOI 5. ICP0, ICP4, and ICP8 gene transcripts were detected by RT-qPCR at 0, 3, 6, and 9 h post-infection (hpi). *P < 0.05