No | St19pv | VSVpv |
---|
Serum (IC50) |
#6 |  × 1600 |  × 25 |
#7 |  >  × 6400 |  × 50 |
#12 |  × 1600 |  × 25 |
#20 |  × 3200 |  <  × 25 |
#21 |  × 400 |  <  × 25 |
#22 |  × 200 |  <  × 25 |
#23 |  × 400 |  <  × 25 |
Whole blood (IC50) |
#6 |  × 1600 |  × 100 |
#7 |  >  × 6400 |  × 100 |
#12 |  × 1600 |  × 100 |
#20 |  × 1600 |  × 100 |
#21 |  × 800 |  × 100 |
#22 |  × 400 |  × 100 |
#23 |  × 400 |  × 100 |
- The pseudotyped viruses, St19pv or VSVpv, were preincubated with serial dilutions (× 25, × 50, × 100, × 200, × 400, × 800, × 1600, × 3200, × 6400) of serum or whole blood from seven different COVID-19 hospitalized patients (patients #6, #7, #12, #20, #21, #22, and #23) with culture media. Thereafter, Vero cells were infected with pseudotyped viruses. Infectivities of pseudotyped viruses were determined by measuring luciferase activities 24 h post-infection. IC50 value was calculated and determined as shown in the dilution range. The results are from three independent assays with error bars representing standard deviations