Table 1 Study population characteristics and results of Multiplex Real-time PCR and Metagenomics approach (n = 94)

1

1 yr

F

Rotavirus A

1

Rotavirus

26.48

2

2 yr

F

–

–

3

3 yr

M

–

–

4

2 mo

M

–

–

5

2 mo

M

–

–

6

6 yr

M

Human adenovirus E

4

–

7

2 yr

F

–

–

8

2 yr

M

Astrovirus MLB2

4

Human adenovirus

25.73

9

1 mo

M

–

–

10

2 yr

M

–

–

11

2 yr

M

Human adenovirus E

1

Human adenovirus

31.99

12

1 yr

M

–

Rotavirus

14.53

Human adenovirus

28.68

13

3 yr

M

–

–

14

3 yr

M

–

–

15

7 mo

M

–

Norovirus GII

16.33

16

3 yr

M

–

–

17

1 yr

M

–

Rotavirus

18.61

Human adenovirus

30.20

Sapporo virus

33.30

Norovirus GII

28.57

18

1 yr

M

Human adenovirus C

12

Human adenovirus

23.96

Human adenovirus E

2

Norovirus GII

17.33

Norovirus GII

11

19

4 yr

F

Enterovirus C

587

–

Enterovirus B

452

Enterovirus D

1

20

3 yr

M

–

Rotavirus

25.90

Human adenovirus

20.05

21

2 yr

F

Norovirus GII

2

Rotavirus

31.62

Human adenovirus C

1

Human adenovirus

33.00

22

1 yr

M

Primate bocaparvovirus-1

1

Rotavirus

32.65

23

1 yr

M

–

–

24

4 yr

M

–

–

25

1 yr

M

Human adenovirus C

1

Rotavirus

26.36

26

2 mo

F

–

–

27

3 mo

M

Human enterovirus

2

–

28

3 mo

M

–

–

29

1 yr

M

Enterovirus B

3

–

Sample No.

Age

Gender

Metagenomics sequencing results

No. of Reads

Multiplex Real-Time PCR results

CT value

30

4 mo

M

Human adenovirus F

744

Human adenovirus

12.31

Human adenovirus C

47

Human adenovirus B

9

31

3 yr

F

–

–

32

4 yr

M

Human adenovirus F

550

Human adenovirus

10.62

Human adenovirus B

12

Human adenovirus E

4

Human adenovirus C

4

Human adenovirus D

4

33

2 yr

M

Rotavirus A

41

Rotavirus

16.40

Human adenovirus C

Human adenovirus

26.55

1

34

2 yr

M

Astrovirus MLB2

1272

Human adenovirus

32.47

Human enterovirus

1

Norovirus GII

24.99

Enterovirus A

1

35

4 yr

F

–

Rotavirus

30.78

Human adenovirus

27.83

36

2 yr

M

Human adenovirus B

1

Human adenovirus

21.38

37

5 yr

F

Human adenovirus F

2

–

38

4 yr

M

–

–

39

1 yr

M

Rotavirus A

5

Rotavirus

14.65

Human enterovirus

1

Human adenovirus

24.82

Husavirus

2

Norovirus GII

1

40

8 mo

M

Human adenovirus F

436

Human adenovirus

12.48

Human adenovirus C

9

41

4 yr

M

Human adenovirus B

1

Rotavirus

32.64

Husavirus

1

Human adenovirus

19.34

42

4 yr

M

–

–

43

7 mo

F

–

Rotavirus

14.83

Human adenovirus

32.30

44

2 yr

M

Rotavirus A

3

Rotavirus

16.99

Human adenovirus B

1

Human adenovirus

32..82

45

2 yr

F

Cardiovirus (saffold)

8

Human astrovirus

32.51

Human adenovirus E

1

Human adenovirus

32.88

Torque teno virus-3

4

Torque teno virus-19

1

46

4 yr

M

–

Human adenovirus

26.44

47

2 yr

F

–

Rotavirus

19.81

Human adenovirus

28.67

Sample No.

Age

Gender

Metagenomics sequencing results

No. of Reads

Multiplex Real-Time PCR results

CT value

48

2 yr

F

Rotavirus A

105

Rotavirus

22.31

49

3 yr

F

–

Rotavirus

29.60

Human adenovirus

29.52

50

1 mo

M

–

–

51

4 mo

M

–

–

52

2 yr

F

Rotavirus A

2

Rotavirus

16.72

Human adenovirus

29.55

53

1 yr

M

–

–

54

1 yr

M

–

Rotavirus

32.55

55

5 yr

M

Rotavirus A

2

Rotavirus

12.85

Human adenovirus

32.62

56

1 yr

F

–

Rotavirus

21.22

Human adenovirus

32.30

57

10 mo

M

Rotavirus A

40

Rotavirus

17.84

58

2 mo

F

Human adenovirus A

1

Rotavirus

32.22

59

1 yr

M

Norovirus GII

10

Norovirus GII

15.87

Parechovirus A

1

Sapporo virus

18.42

Human adenovirus

32.51

60

1 yr

M

Rotavirus A

4

Rotavirus

16.78

Human adenovirus B

1

Human adenovirus

26.70

Aichi virus A

1

Norovirus GII

27.57

61

3 yr

M

Primate bocaparvovirus 1

6

Rotavirus

16.34

Human adenovirus B

1

Human adenovirus

29.83

Human adenovirus G

1

Norovirus GII

28.49

Norovirus GII

1

62

8 mo

M

–

Human adenovirus

32.87

Norovirus GII

31.84

63

3 yr

M

–

–

Rotavirus

20.27

Human adenovirus

30.71

Norovirus GII

28.94

64

1 yr

M

Rotavirus A

13

Rotavirus

18.31

65

3 yr

F

Astrovirus VA4

2

Human adenovirus

32.63

Torque teno virus

1

Norovirus GI

30.00

66

2 yr

M

Rotavirus A

5

Rotavirus

20.58

Parainfluenza virus 1

10

67

4 yr

M

–

–

68

7 yr

F

Human adenovirus B

5

–

69

1 mo

F

–

–

–

70

3 mo

M

–

–

–

71

3 yr

M

–

–

–

72

6 yr

M

Cosavirus F

1

Human adenovirus

31.84

Bufavirus-3

1

73

4 yr

F

Norovirus GII

1

–

Sample No.

Age

Gender

Metagenomics sequencing results

No. of Reads

Multiplex Real-Time PCR results

CT value

74

1 yr

M

Rotavirus A

18

Rotavirus

14.76

Human adenovirus

29.54

Sapporo virus

27.25

75

3 mo

M

–

–

–

76

1 mo

M

–

Human adenovirus

33.00

77

4 mo

M

Enterovirus C

11

–

78

1 yr

F

–

–

Human astrovirus

31.73

79

5 mo

F

Norovirus GII

3

Norovirus GII

16.96

80

6 mo

M

Norovirus GII

621

Norovirus GII

13.57

Human adenovirus

32.77

81

2 yr

F

Human adenovirus C

159

Rotavirus

30.97

Norovirus GII

26

Human adenovirus

23.34

Norovirus GII

16.51

82

2 yr

F

Norovirus GII

5

Norovirus GII

19.25

83

2 yr

M

Norovirus GII

33

Norovirus GII

16.88

84

5 yr

M

–

–

Rotavirus

19.36

85

2 yr

F

–

–

–

86

1 yr

M

–

–

–

87

3 yr

F

–

–

–

88

5 yr

M

Husavirus

1

–

89

4 yr

M

–

–

–

90

2 yr

M

–

–

–

91

4 yr

M

Human adenovirus C

1

Rotavirus

16.52

Human adenovirus

31.35

92

4 yr

F

–

–

–

93

4 yr

M

Rotavirus A

3

Rotavirus

16.44

94

2 yr

M

Astrovirus MLB2

1

–