Fig. 6
From: Identification of cellular microRNA miR-188-3p with broad-spectrum anti-influenza A virus activity
miR-188-3p inhibits the replication of IAV through targeting the potential binding sites on PB2. a specific miRNA inhibitors were contransfected into HEK-293 T cells with the miRNA mimics and dual-luciferase reporter vectors to further confirm the inhibitory effect of miR-188-3p on PB2 expression. b the potential binding sites of miR-188-3p seed region were mutant in the luciferase reporter vector. c Luciferase assay to analyze the importance of binding sites of miR-188-3p in the PB2 gene to inhibit the relative luciferase activity from pGL3-PB2–188-3p and pRL-TK-control vector. * (P < 0.05), **(P < 0.01), ***(P < 0.001), results were significantly different from negative control miRNA group