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Table 4 Comparison of test results and return results of inter-laboratory quality assessment panel

From: Analysis and validation of a highly sensitive one-step nested quantitative real-time polymerase chain reaction assay for specific detection of severe acute respiratory syndrome coronavirus 2

Sample ID

Test results

Return results

qRT-PCR (Ct value)

OSN-qRT-PCR (Ct value)

ddPCR (copies/mL)

SARS-COV-2

Pass rate (%)

ORF-1ab

N

ORF-1ab

N

ORF-1ab

N

202001

Negative

99

202002

31.79

30.49

16.45

20.97

3074

2073

Positive

98

202003

25.99

24.23

13.78

18.68

23,080

5005

Positive

99

202004

33.87

32.31

20.94

21.19/26.67

1144

2860

Positive

98

202005

29.93

28.87

15.56

19.53

7293

2072

Positive

99

202006

Negative

99

202007

32.85

31.73

18.38

20.80

1144

1573

Positive

98

202008

Negative

99

202009

34.85

33.91

18.92

22.43

1312

643

Positive

97

202010

27.79

26.40

12.22

15.89

33,962

11,072

Positive

99