Multiple gene targeting siRNAs for down regulation of Immediate Early-2 (Ie2) and DNA polymerase genes mediated inhibition of novel rat Cytomegalovirus (strain All-03)

Table 3 Primers and probe sequences used for real-time PCR assay

Target	Primer sequence	Product size (bp)
GAPDH
Forward	tctccaccactatcgcagaa	100
Reverse	ttggcagcttggactatgct
Probe	FAM-tccgttttggcagagaagatgcaa-TAMRA
ie2b
Forward	gcgattttgatctacgtg	256
Reverse	acagcgaacctatagaca
Probe	FAM-caacggggggaggaaacaga-TAMRA
dpb
Forward	aggacatcatcacgagaa	100
Reverse	tcaaacaaagatagcggg
Probe	FAM-agatcggtggggtatcagg-TAMRA
dpc
Forward	ccgaagtaccagattcaa	102
Reverse	gacgagagggagtatatt
Probe	FAM-cggacggtgaactcgttttt-TAMRA

ISSN: 1743-422X