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Fig. 2 | Virology Journal

Fig. 2

From: A ligation and restriction enzyme independent cloning technique: an alternative to conventional methods for cloning hard-to-clone gene segments in the influenza reverse genetics system

Fig. 2

The PB1 positive colonies of Bangladesh/2014 and Vietnam/2014 H9N2 virus were screened by plasmid PCR using the PB1 primers [31]. Out of 16 colonies screened for Bangladesh PB1 (lane 1–16), 3 were positive (13, 14, 15; indicated by arrows) while for 9 colonies screened for Vietnam PB1 (lane 17–25), 4 were found to be positive (22, 23, 24, 25; indicated by arrows). The positive clones were further confirmed by nucleotide sequencing using T7F and BGHR primers. (+ = positive control; M = DNA marker)

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Virology Journal

ISSN: 1743-422X