HBV region | Round | Primer Name (Position) | Primer Sequence (5′-3′) | Thermal Profile |
---|---|---|---|---|
BCP/Precore region | First | BcpF1(1254–1273) | TCCTCTGCCGATCCATACTG | 80 °C(3 min); 95 °C (40 s), 63 °C (1 min), 71 °C (2.5 min)/40 cycle; 72 °C(7 min) |
BcpR1(2416–2439) | TTCCCGAGATTGAGATCTTCTGCG | |||
Second | BcpF2(1606–1625) | GCATGGAGACCACCGTGAAC | 95 °C(5 min); 95 °C (40 s), 50 °C (30 s), 72 °C (1 min)/35 cycle; 72 °C(7 min) | |
BcpR2(1955–1974) | GGAAAGAAGTCAGAAGGCAA | |||
Complete genome (without BCP) | First | HBV1799FLong(1799–1826) | CTGCGCACCAGCACCATGCAACTTTTTC | 80 °C (3 min); 95 °C (40 s), 58 °C (1.5 min), 68 °C (4 min)/45 cycle; 72 °C(7 min) |
HBV1801RLong(1774–1801) | CAGACCAATTTATGCCTACAGCCTCCTA | |||
Second | Â | Â | Â | |
1 | HBV1847FS (1847–1867) | TGTTCATGTCCCACTGTTCAA | 95 °C(5 min); 95 °C (40 s), 63 °C (30 s), 72 °C (1 min)/35 cycle; 72 °C(7 min) | |
HBV2394RS (2394–2408) | GGCGAGGGAGTTCTT | |||
2 | HBV2298FS (2298–2315) | GACCACCAAATGCCCCTAT | ||
HBV2933RS (2933–2954) | TCGGGAAAGAATCCCAGAGGAT | |||
3 | HBV2821FS (2821–2840) | GGTCACCATATTCTTGGGAAC | ||
HBV272RS (272–291) | TGAGAGAAGTCCACCACGAGT | |||
4 | HBV179FS (179–197) | CTAGGACCCCTGCTCGTGTT | ||
HBV704RS (704–725) | CGAACCACTGAACAAATGGCACT | |||
5 | HBV0599FS(599–619) | GTATTCCCATCCCATCATCCTG | ||
HBV1286RS (1286–1305) | GCTAGGAGTTCCGCAGTATGG | |||
6 | HBV1175FS (1175–1190) | GCCAAGTGTTTGCTGA | ||
HBV1788RS(1788–1802) | GCCTACAGCCTCCTA | |||
SP6 | CGATTTAGGTGACACTATAGAAGAGAGGCT | Â | ||
T7 | CAGTAATACGACTCACTATAGGGAGAAGGCT | Â |